• University of Southampton Featured Masters Courses
  • Jacobs University Bremen gGmbH Featured Masters Courses
  • University of Bristol Featured Masters Courses
  • University of Oxford Featured Masters Courses
  • Goldsmiths, University of London Featured Masters Courses
  • Swansea University Featured Masters Courses
  • University of Derby Online Learning Featured Masters Courses
  • University of Edinburgh Featured Masters Courses
Cranfield University Featured Masters Courses
OCAD University Featured Masters Courses
University of Reading Featured Masters Courses
Barcelona Technology school Featured Masters Courses
University of Leeds Featured Masters Courses
"enzymology"×
0 miles

Masters Degrees (Enzymology)

We have 9 Masters Degrees (Enzymology)

  • "enzymology" ×
  • clear all
Showing 1 to 9 of 9
Order by 
Application period/deadline. November 1, 2017 - January 24, 2018. In-depth training in understanding structure-function relationships of proteins and their characterisation. Read more

Application period/deadline: November 1, 2017 - January 24, 2018

• In-depth training in understanding structure-function relationships of proteins and their characterisation

• Strong focus on practical skills and use of most modern equipment in protein expression and analysis

• Highly flexible degree aimed at students with an interest in a research career, taught by an international staff

The International Master’s Degree Programme in Protein Science and Biotechnology is a two-year programme planned around the early integration of M.Sc. students into research groups and the hands-on use of modern biochemical and molecular biology equipment by individual students. Early exposure to research work provides insights into cutting edge approaches in structural and enzymology characterisation as well as cell and molecular biology methods. A completion of a minimum of 120 study units equivalent to ECTS credits is required to complete the master’s degree studies. The flexible programme includes courses in:

• Protein production and analysis (compulsory)

• Biochemical methodologies (compulsory)

• 3-6 week orientation to research work periods in research groups (compulsory)

• Basic aspects of crystallographic methods

• Structural enzymology

• Biochemistry of protein folding

• Systems biology

• Bioinformatics and biocomputing

• Structure-based drug discovery

Additional optional studies include (but are not limited to):

• Advanced biotechnology/bioprocess engineering

• Immunology

• Animal use in research

• Yeast genetics and genomics

• Information skills for foreign degree students

• Bioreactor technology

• Molecular bases of disease

In addition, up to 15 credits can be taken from other suitable courses taught at the Oulu University or any other university, as long as they are of the appropriate level and connected to biochemistry or logically support some aspect of the Protein Science and Biotechnology programme.

Due to the range of courses available in the programme, a wide variety of expertise that can be obtained during M.Sc. level studies at FBMM. The official diploma title received after successful completion of our international M.Sc. programme will be M.Sc. in Protein Science and Biotechnology. Depending on the course choices, the training received may also provide you with excellent proficiency in molecular and cellular biology.

The duration of the M.Sc. thesis research work is flexible depending on the interest of the students and may be three months (more courses/lectures taken) or eight months (longer M.Sc. thesis research period).

Significant number of students spend orientation to research work periods outside the Faculty of Biochemistry and Molecular Medicine or carry out the research work for their MSc thesis abroad

The Faculty of Biochemistry and Molecular Medicine offers a highly international environment of cutting edge research in Protein Structure analysis, Enzymology, Proteomics, Bioimaging, Developmental Biology, Matrix Biology and Metabolism research. About fifty percent of our staff are native to other countries than Finland, and research groups are well connected globally to other specialists and research groups in their fields of study. Many students holding an M.Sc. from our faculty have gone on to Ph.D. programmes of other prestigious institutions all over the world, and many have stayed at FBMM Oulu to continue in our Ph.D. programme.

The skills gained in the programme offer you the academic training and expertise required to succeed in a research environment, but will also open opportunities in biomedical and related industries.

Successful applicants should hold a B.Sc. or higher degree in Biochemistry, Chemistry or a related field in the natural or life sciences and have a good command of technical English language in biochemistry and molecular biology.

Email Now



Read less
The chemistry of biological processes is the basis of all life on planet Earth. On this course you will develop an understanding of the processes that are core to biological chemistry. Read more
The chemistry of biological processes is the basis of all life on planet Earth. On this course you will develop an understanding of the processes that are core to biological chemistry. We will explore aspects such as biosynthesis, retrosynthetic analysis, molecular biology and the principles of drug development. We will also look at the applications of biological chemistry in catalysts, synthetic methods and spectroscopy, giving our graduates an edge when looking for employment in academia or industry.

Distinctive features:

• Available on a one year full-time or three year part-time basis.

• Explore real life biological systems as well as applications of biological processes, for example in catalysis.

• Specialise in an area of interest to you with an end of course research project.

• Some overseas academic placements may be available for the research project.

Structure

This course may be taken on a one year full-time or three year part-time basis.

There are two parts to the degree. Part one comprises core and optional taught modules which you will take during the autumn and spring semesters. In these modules we will provide you with an understanding of the biological problems and processes at the interface of chemistry and biology. We will study real life systems and explore aspects such as natural product synthesis, biocatalysis, molecular biology, synthetic biology, enzymology, medicinal chemistry and molecular modelling.

Upon successful completion of part one of the degree you will progress to part two, the summer research project. We will make a range of project options available to you from the field of biological chemistry. For this project you may work with a research group in the School of Chemistry. You may also be able to complete this project with one of our academic partner institutions overseas.

If you are on the one year full-time degree option, you will undertake all modules and your research project in one year.

Core modules:

Structure and Mechanism in Organic Chemistry
Biosynthetic Approach to Natural Products
Biocatalysis I - Modern Approaches to Biocatalysts
Colloquium
Biocatalysis II - Industrial Applications of Biocatalysis
Medicinal Chemistry
Bioinorganic Chemistry
Advanced Techniques in Organic and Biological Chemistry
Key Skills for Postgraduate Chemists
Practical Chemical Biology
Research Project

Optional modules:

Modelling of Biological Macromolecules
Asymmetric Synthesis of Pharmaceuticals and Natural Products
Analytical and Structural Techniques in Chemical Biology
Molecular Modelling

Teaching

The methods of teaching we employ will vary from module to module, as appropriate depending on the subject matter and the method of assessment. We teach using a mixture of lectures, workshops, computational sessions, laboratory practicals and tutorials.

Your research project will be carried out in one of our laboratories under supervision of an academic member of staff with interests in a similar field, unless you choose to complete your project during a placement with one of our academic partner institutions overseas, depending on availability.

Modules relating to computing frequently take place in our computer rooms, while practical work will be undertaken in our laboratories. We frequently invite external academic speakers and industry experts to the School for seminars, which our postgraduate students are encouraged to attend.

Support

All of our students are allocated a personal tutor when they enrol on the course. A personal tutor is there to support you during your studies and can advise you on academic and personal matters that may be affecting you. You should have regular meetings with your personal tutor to ensure that you are fully supported.

You will have access to the Science Library, which holds our collection of chemistry resources, as well as to the other Cardiff University Libraries.

Feedback:

We offer written and oral feedback, depending on the coursework or assessment you have undertaken. You will usually receive your feedback from the module leader. If you have questions regarding your feedback, module leaders are usually happy to give advice and guidance on your progress. We aim to provide you with regular feedback on your work after assessments have been submitted.

Assessment

Taught modules are assessed in a variety of ways depending on the module content and learning outcomes (found in the module descriptions). We use course work, assessed workshops, posters and oral presentations or a combination of these to assess your progress on the course.

Your research project at the end of the course will be assessed through a dissertation, a presentation, and an oral exam.

Career prospects

After completing this course there are usually two career streams open to graduates, research or industry. Within these two fields there are a variety of career options. For example, many of our graduates choose to follow up their MSc and decide to complete a PhD research degree with us. Those who have chosen not to continue in academia or teaching have gone on to a wide range of employment in private industries such as Kimberley-Clark group, Thales group, and Imanova Ltd.

Placements

For the end of course research project we may have some placements available with one of our academic partner institutions overseas. Please enquire early for further details

Read less
Biotechnology is defined as the industrial exploitation of living organisms or the exploitation of components derived from these organisms. Read more

MSc Biotechnology

Biotechnology is defined as the industrial exploitation of living organisms or the exploitation of components derived from these organisms.

Programme summary

During the master Biotechnology you learn more about the practical applications of biotechnology, including age-old techniques such as brewing and fermentation, which are still important today. In recent decades, gene modification has revolutionized the biotechnology industry, spawning countless new products and improving established processes. Modern biotechnology has become an applied area of science with a multidisciplinary approach embracing recombinant DNA technology, cellular biology, microbiology, biochemistry, as well as process design and engineering.

Specialisations

Cellular and Molecular Biotechnology
This specialisation focuses on the practical application of cellular and molecular knowledge with the aim of enhancing or improving production in micro-organisms or cell cultures. Possible majors: molecular biology, biochemistry, microbiology, virology, enzymology and cell biology. The knowledge and skills gained can be applied in food biotechnology, medicine and vaccine development, environmental and bio-based technology.

Process Technology
This specialisation focuses on engineering strategies for developing, enhancing or improving production in fermentation, bioconversion and enzymatic synthesis. Possible majors: bioprocess engineering, food or environmental engineering, applied biotechnology and system and control techniques. The knowledge and skills gained can be applied in food biotechnology, medicine and vaccine development, environmental and bio-based technology.

Marine Biotechnology
This specialisation focuses on the use of newly- discovered organisms from the sea in industrial processes. Applications include production of new medicines, fine chemicals, bio-based products and renewable energy.

Medical Biotechnology
This specialisation focuses on the use of modern biotechnology in the development and production of new vaccines and medicines. Advanced molecular and cellular techniques are used to study diagnostic and production methods for vaccines and medicines. Possible majors: molecular biology, microbiology, virology and cell biology.

Food Biotechnology
This specialisation focuses on the application from biotechnology to food processing. The approach includes microbial and biochemical aspects integrated with process engineering and chemistry. Possible majors: food microbiology, food chemistry and process engineering.

Environmental and Biobased Technology
This specialisation focuses on the design and development of biotechnological processes for solving environmental problems by removing waste products or by producing renewable energy. Possible majors: environmental technology, bioprocess engineering, microbiology and biobased chemical technology.

Your future career

Graduates in biotechnology have excellent career prospects. More than 60 percent begin their careers in research and development. Many of these Master graduates go on to earn their PhD degrees and often achieve management positions within a few years. Approximately 30 percent of our graduates start working for biotechnology companies immediately. Relatively few begin their careers outside the private sector or in a field not directly related to biotechnology. In the Netherlands, some graduates work for multinational companies such as Merck Schering Plough, DSM, Heineken, Unilever and Shell, while others find positions at smaller companies and various universities or research centres such as NKI and TNO.

Alumnus Sina Salim.
In America and Brazil, production of maize and sugar cane for bio ethanol takes up enormous swathes of arable land that could otherwise be used for food production. This leads to the well-known food versus fuel dilemma. An alternative method for producing biodiesel is the use of algae. Currently, too much energy is consumed during the growth and harvesting of algae, but huge efforts are being made to reduce these energy requirements. Sina Salim is trying to develop a cheap and energy efficient harvesting method to ultimately produce biodiesel from algae, a competitor of fossil fuel. Now he is operational scientist at Bioprocess Pilot Facility B.V.

Related programmes:
MSc Molecular Life Sciences
MSc Food Technology
MSc Bioinformatics
MSc Plant Biotechnology
MSc Environmental Sciences.

Read less
The Molecular Life Sciences programme focuses on the molecular aspects of the fields of nutrition, health, nature and the living environment and works in close coordination with colleagues from different disciplines. Read more

MSc Molecular Life Sciences

The Molecular Life Sciences programme focuses on the molecular aspects of the fields of nutrition, health, nature and the living environment and works in close coordination with colleagues from different disciplines.

Programme summary

The Molecular Life Sciences programme focuses on molecules and their properties. It seeks to discover relationships between the physical and chemical properties of molecules, particularly the role of complex molecules in living systems. It is an interdisciplinary programme that combines chemistry, physics and biology. The aim of the programme is to enable students to conduct independent research at the interface of chemistry, biology and physics, or in an applied field such as medicine, the environment, food sciences or (bio) nanotechnology. The programme is tailormade and thesis-oriented, with the thesis being the culmination of the study.

Specialisations

Biological Chemistry
By combining the principles of chemistry, biochemistry, molecular biology, cell biology, microbiology, genetics and bioinformatics, this specialisation enables students to contribute new insights to the life sciences. Increasingly complex areas are studied, such as the molecular regulation of growth and cell differentiation, gene control during development and disease, and the transfer of genetic traits. Another important field is enzymology where enzyme mechanisms are studied with the aim of understanding and modifying their properties to make new compounds or biological membranes.

Physical Chemistry
This specialisation uses the most advanced technologies to focus on the chemical and physical properties of molecules and their behaviour in chemical and biochemical processes. The processes in nature are used as models for studying and synthesising new compounds with interesting chemical or physical properties for applications such as LCDs, biosensors or food science. Students can major in the fields of biophysics, organic chemistry or physical chemistry and colloid science.

Biomedical Research
This specialisation equips graduates with key skills in the natural sciences and enables them to use these skills as part of an integrated approach. Many recent breakthroughs in biomedical research have taken place at the interface between chemistry, biology and physics, so it is logical that many of our graduates enter careers in biomedical research. The explicit aim of this specialisation is to prepare students for careers at a medical research institute, academic hospital or a company in the pharmaceutical industry. As a result, students also complete their internships at such locations.

Physical Biology
Students in this specialisation learn to view biomolecules from a physical point of view. They use techniques in biophysics, physical chemistry, microspectroscopy and magnetic resonance (MRI) to contribute to areas such as cell-cell communication, transformation of light into chemical energy, and protein interactions. Students can major in fields such as biochemistry, biophysics, microbiology, molecular biology, plant physiology, physical chemistry and colloid science.

Your future career

By combining the power of chemistry, physics and biology, graduates are able to make a significant contribution to fundamental and/or applied research in fields such as (bio) nanotechnology, biotechnology, environmental research, biomedical research, nutrition and the food sciences. Our graduates enter careers at universities, research institutes and industrial laboratories. The first job for many of our graduates is a four year PhD project at a university or research institute. This is not only an excellent preparation for a research career, but it also prepares you for management positions. Others become science journalists, teachers or consultants in government or industry.

Project Flu Vaccination for bacteria.
Together with his colleagues of the Laboratory of Microbiology, professor John van der Oost unravelled part of the working of the immune systems of bacteria that had been infected by a virus. Theoretically, this knowledge allows for other bacteria to be protected against specific viruses and, thus, may be considered to be a flu vaccination for bacteria. Understanding this process in simple organisms on a molecular level, is the first step in revealing the mechanism of viral infection in the human body. This can be the starting point for a whole new line of medicines.

Related programmes:
MSc Biotechnology
MSc Food Technology
MSc Bioinformatics
MSc Nutrition and Health
MSc Plant Biotechnology
MSc Biology

Read less
Programme description. Changing demographics and growing demand for food, fuel and agricultural and environmental sustainability are among the key challenges the world faces today. Read more

Programme description

Changing demographics and growing demand for food, fuel and agricultural and environmental sustainability are among the key challenges the world faces today.

In this MSc you will learn research and development skills to enable the creation of new products and services. You will investigate the economic basis for current biotechnology structures and areas of future demand, including the global pharmaceutical industry and carbon sequestration.

You will learn how technology can be applied to solve pressing real-world biological problems and gain the skills and expertise needed for future developments in biotechnology.

Programme structure

This programme consists of two semesters of taught courses followed by a research project or industrial placement, leading to a dissertation.

Compulsory courses:

  • Economics and Innovation in the Biotechnology Industry
  • Intelligent Agriculture
  • Principles of Industrial Biotechnology
  • Research Project Proposal
  • MSc Dissertation (Biotechnology).

Option courses:

  • Biobusiness
  • Biochemistry A & B
  • Bioinformatics
  • Bioinformatics Programming & System Management
  • Drug Discovery
  • Commercial Aspects of Drug Discovery
  • Environmental Gene-Mining and Metagenomics
  • Enzymology and Biological Production
  • Gene Expression and Microbial Regulation
  • Industry & Entrepreneurship in Biotechnology
  • Molecular Modelling and Database Mining
  • Practical Skills in Biochemistry A & B
  • Programming for the Life Sciences
  • Social Dimensions of Systems and Synthetic Biology
  • Stem Cells and Regenerative Medicine
  • Vaccines and Molecular Therapies

Research and laboratory work

There will be a considerable practical element to the programme. You will work in a biotechnology laboratory and learn how experimental technology is designed and operated.

Industrial placement

Your dissertation can be based on a laboratory-based project or an industrial placement. You can work with employers in the thriving Scottish biotechnology sector in areas such as multiple sclerosis research (Aquila BioMedical), vaccines research (BigDNA) or biorecovery and bioregeneration (Recyclatec).

Career opportunities

The programme will open up a wide variety of career opportunities, ranging from sales and marketing, to research and development, to manufacturing and quality control and assurance.



Read less
This academically challenging and career-developing programme focuses on research and development using biological and chemical principles and systems to create new products, services and industries. Read more

This academically challenging and career-developing programme focuses on research and development using biological and chemical principles and systems to create new products, services and industries.

You will employ elements of the developing field of synthetic biology to bring about significant changes and major innovations that address the challenges of rapidly changing human demographics, resource shortages, energy economy transition and the concomitant growth in demand for more and healthier food, sustainable fuel cycles, and a cleaner environment.

Programme structure

You will learn through a variety of activities, including:

  • lectures
  • workshops
  • presentations
  • laboratory work
  • field work
  • tutorials
  • seminars
  • discussion groups and project groups
  • problem-based learning activities

You will attend problem-based tutorial sessions and one-to-one meetings with your personal tutor or programme director.

You will carry out research at the frontier of knowledge and can make a genuine contribution to the progress of original research. This involves carrying out project work in a research laboratory, reviewing relevant papers, analysing data, writing reports and giving presentations.

Compulsory courses:

  • Applications of Synthetic Biology
  • Tools for Synthetic Biology
  • Social Dimensions of Systems & Synthetic Biology
  • Environmental Gene Mining & Metagenomics
  • Research Project Proposal
  • MSc Project and Dissertation

Option courses:

  • Biochemistry A & B
  • Introduction to Scientific Programming
  • Commercial Aspects of Drug Discovery
  • Stem Cells & Regenerative Medicine
  • Biological Physics
  • Enzymology & Biological Production
  • Next Generation Genomics
  • Machine Learning & Pattern Recognition
  • Drug Discovery
  • Biophysical Chemistry
  • Bioinformatics Programming & System Management
  • Economics & Innovation in the Biotechnology Industry
  • BioBusiness
  • Molecular Modelling & Database Mining
  • Industry & Entrepreneurship in Biotechnology
  • Practical Skills in Biochemistry A & B
  • Functional Genomic Technologies
  • Information Processing in Biological Cells
  • Data Mining & Exploration
  • Gene Expression & Microbial Regulation
  • Bioinformatics
  • Principles of Industrial Biotechnology

Learning outcomes

By the end of the programme you will have gained:

  • a strong background knowledge in the fields underlying synthetic biology and biotechnology
  • an understanding of the limitations and public concerns regarding the nascent field of synthetic biology including a thorough examination of the philosophical, legal, ethical and social issues surrounding the area
  • the ability to approach the technology transfer problem equipped with the skills to analyse the problem in scientific and practical terms
  • an understanding of how biotechnology relates to real-world biological problems
  • the ability to conduct practical experimentation in synthetic biology and biotechnology
  • the ability to think about the future development of research, technology, its implementation and its implications
  • a broad understanding of research responsibility including the requirement for rigorous and robust testing of theories and the need for honesty and integrity in experimental reporting and reviewing

Career opportunities

You will enhance your career prospects by acquiring current, marketable knowledge and developing advanced analytical and presentational skills, within the social and intellectual sphere of a leading European university.

The School of Biological Sciences offers a research-rich environment in which you can develop as a scientist and entrepreneur.



Read less
. Research profile. Pursuing a research degree at the School of Chemistry could be one of the best experiences of your life. Read more

Research profile

Pursuing a research degree at the School of Chemistry could be one of the best experiences of your life.

In addition to gaining research skills, making friends, meeting eminent researchers and being part of the research community, a research degree will help you to develop invaluable transferable skills which you can apply to academic life or a variety of professions outside of academia.

The Chemistry/Biology Interface

This is a broad area, with particular strengths in the areas of protein structure and function, mechanistic enzymology, proteomics, peptide and protein synthesis, protein folding, recombinant and synthetic DNA methodology, biologically targeted synthesis and the application of high throughput and combinatorial approaches. We also focus on biophysical chemistry, the development and application of physicochemical techniques to biological systems. This includes mass spectrometry, advanced spectroscopy and microscopy, as applied to proteins, enzymes, DNA, membranes and biosensors.

Experimental & Theoretical Chemical Physics

This is the fundamental study of molecular properties and processes. Areas of expertise include probing molecular structure in the gas phase, clusters and nanoparticles, the development and application of physicochemical techniques such as mass spectoscropy to molecular systems and the EaStCHEM surface science group, who study complex molecules on surfaces, probing the structure property-relationships employed in heterogeneous catalysis. A major feature is in Silico Scotland, a world-class research computing facility.

Synthesis

This research area encompasses the synthesis and characterisation of organic and inorganic compounds, including those with application in homogeneous catalysis, nanotechnology, coordination chemistry, ligand design and supramolecular chemistry, asymmetric catalysis, heterocyclic chemistry and the development of synthetic methods and strategies leading to the synthesis of biologically important molecules (including drug discovery). The development of innovative synthetic and characterisation methodologies (particularly in structural chemistry) is a key feature, and we specialise in structural chemistry at extremely high pressures.

Materials Chemistry

The EaStCHEM Materials group is one of the largest in the UK. Areas of strength include the design, synthesis and characterisation of functional (for example magnetic, superconducting and electronic) materials; strongly correlated electronic materials, battery and fuel cell materials and devices, porous solids, fundamental and applied electrochemistry polymer microarray technologies and technique development for materials and nanomaterials analysis.

Training and support

Students attend regular research talks, visiting speaker symposia, an annual residential meeting in the Scottish Highlands, and lecture courses on specialised techniques and safety. Students are encouraged to participate in transferable skills and computing courses, public awareness of science activities, undergraduate teaching and to represent the School at national and international conferences.

Facilities

Our facilities are among the best in the world, offering an outstanding range of capabilities. You’ll be working in recently refurbished laboratories that meet the highest possible standards, packed with state-of-the-art equipment for both analysis and synthesis.

For NMR in the solution and solid state, we have 10 spectrometers at field strengths from 200-800 MHz; mass spectrometry utilises EI, ESI, APCI, MALDI and FAB instrumentation, including LC and GC interfaces. New combinatorial chemistry laboratories, equipped with a modern fermentation unit, are available. We have excellent facilities for the synthesis and characterisation of bio-molecules, including advanced mass spectrometry and NMR stopped-flow spectrometers, EPR, HPLC, FPLC, AA.

World-class facilities are available for small molecule and macromolecular X-ray diffraction, utilising both single crystal and powder methods. Application of diffraction methods at high pressures is a particular strength, and we enjoy strong links to central facilities for neutron, muon and synchrotron science in the UK and further afield. We are one of the world's leading centres for gas-phase electron diffraction.

Also available are instruments for magnetic and electronic characterisation of materials (SQUID), electron microscopy (SEM, TEM), force-probe microscopy, high-resolution FTRaman and FT-IR, XPS and thermal analysis. We have also recently installed a new 1,000- tonne pressure chamber, to be used for the synthesis of materials at high pressures and temperatures. Fluorescence spectroscopy and microscopy instruments are available within the COSMIC Centre. Dedicated computational infrastructure is available, and we benefit from close links with the Edinburgh Parallel Computing Centre.



Read less
Tralee is currently seeking to recruit a high calibre and suitably qualified science graduate to undertake this Master of Research programme in the Department of Biological and Pharmaceutical Sciences at IT Tralee. Read more

Tralee is currently seeking to recruit a high calibre and suitably qualified science graduate to undertake this Master of Research programme in the Department of Biological and Pharmaceutical Sciences at IT Tralee. Graduates holding a relevant Level 8 Honours Degree (second class honours or higher) are invited to submit an application. The successful applicants will be awarded a stipend of €700 per month for a maximum period of 18 months and the Institute will waive full fees for this funding period. Postgraduate students are expected to complete their studies full-time at the Institute.

Biography of Principle Supervisor

Dr Oscar Goñi received his Degree in Chemistry from the University of Navarra (Spain), an MSc in Biochemistry and Molecular Biology from Complutense University of Madrid (Spain) and completed his PhD in Plant Protein Biochemistry at ICTAN-CSIC (Spain) and Complutense University of Madrid (Spain). Dr Goñi has previously worked as a Postdoctoral Research Fellow in the Max Planck Institute of Plant Breeding Research (Cologne). He is a protein biochemist with experience in the purification and characterization of functional proteins, enzymology and development of protein biomarkers. Dr. Goñi currently holds the position of Postdoctoral Researcher with Shannon ABC / Brandon Bioscience and specialises in the development of enzyme activities for the production of macro-algae derived oligosaccharides and chitin/chitosan derived oligosaccharides for crop protection and yield enhancement. 

Research Project Abstract

The United Nations’ and Agriculture Organization predicts that by 2050 the world will need to produce 70 percent more food than it does currently. Along with improving food storage and transport, increasing crop yields is seen as a primary solution. Salinity is one the major environmental stresses affecting crop production, particularly in arid and semi-arid areas. Most of the vegetable crops are salt sensitive, growing poorly in salinized soils due to the accumulation of toxic ions from prolonged irrigation regimes. A meaningful approach to increase crop yield and counteract salt stress would be the use of protein hydrolysate-based biostimulants, which are gaining interest worldwide. Nowadays, more than 90% of the protein hydrolysates market in agriculture is based on products obtained through chemical hydrolysis of proteins from animal origin. The production and use of new vegetable derived-protein hydrolysates with high plant biostimulant activity has become the focus of much research interest due to their lack of plant phytotoxicity, absence of degraded or biologically inactive amino acids or compatibility in the production of food for vegetarians. The commercial partner, Deltagen UK, aims to commercialise protein hydrolysate biostimulants with superior salinity inducing tolerance. The aim of this research is the development of an innovative system to produce protein hydrolysates from the defatted by product meals of flax, lentil and sesame seeds with the ability to biostimulate plant tolerance to salt stress. Novel protein hydrolysates will be produced using a cocktail of suitable proteases, they will be applied to tomato plants (cv. Micro-Tom) in a controlled growth room under salt stress conditions. Treatments will be assessed by comparing classic phenotypical parameters. Plant tissue will also be saved in order to assess other biochemical and molecular parameters such as stress related proteins and osmoprotectant metabolites.

Research Context (Technical Merit & Impact)

The beginning of 21st century is marked by global scarcity of water resources, environmental pollution and increased salinization of soil and water. An increasing human population and reduction in land available for cultivation are two threats for agricultural sustainability. It has been estimated that worldwide 20% of total cultivated and 33% of irrigated agricultural lands are afflicted by high salinity. It has been projected that more than 50% of the arable land would be salinized by the year 2050. Use of optimized farm management practices such as shifting crop rotation or better irrigation systems can ameliorate yield reduction under salinity stress. However, its implementation is often limited because of cost and availability of good water quality. Several salt-tolerant varieties have been released, the overall progress of traditional breeding has been slow and has not been successful, as only few major determinant genetic traits of salt tolerance have been identified. The utilisation of agro-food processing wastes to generate value added products is an extremely convincing argument as it makes commercial and environmental sense. In addition, it is an excellent, demonstrable example of the European circular economy in action, a key objective of the H2020 research programme, turning waste into value and ultimately food for a growing population.

Research Methodology

Three process variables will be studied in order to obtain the maximum degradation of seed proteins: incubation time, temperature and the initial concentration of meal protein. The Response Surface Methodology (RSM) will be used to reduce the cost and duration of experiments and allow for the observation of any interacting factors in the final process response. Amino acid and monosaccharide composition will be determined by sensitive high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) according previous bibliography. Molecular weight distribution of protein hydrolysates will be characterized by protein electrophoresis (SDS-PAGE) and high performance size exclusion chromatography (HPSEC). The plant trials will involve 2 separate sets of experiments under unstressed and salt-stressed conditions respectively. Experiments will be carried out in a growth room with different concentration rates of different protein hydrolysates and the tomato variety Micro-Tom will be used. This extensive factorial experiment will be assessed by fruit yield, fruit quality, chlorophyll (SPAD measurement), MDH content (cell membrane integrity) and levels of protective compounds (proline and soluble carbohydrates). The presence of stress proteins such as HSPs will be determined using immunoblotting techniques (Western blot). RT-qPCR is another advanced laboratory technique that will be emp



Read less
Chemical biology is the application of chemical tools and ideas to biological and medical problems. Read more

Chemical biology is the application of chemical tools and ideas to biological and medical problems. This programme is designed to build on an existing knowledge of chemical structure and reactivity to give you a thorough grounding in contemporary chemical biology and drug discovery as well as introducing you to topics from the research frontier.

You’ll be taught by experts from across the Astbury Centre in chemical biology, biophysics and medicinal chemistry using a "problem-based" approach. Visiting lecturers from the pharmaceutical industry will share their expertise in industrially-relevant applications of chemical biology and drug design with you.

Bridging the gap between your undergraduate degree in a core subject, and interdisciplinary research in chemical biology, you’ll develop the skills to solve real-life research problems, benefitting from a multi-million pound investment in fantastic research facilities. Rather than focusing on a single discipline, you’ll learn to use either chemical or biological approaches to tackle the problem in hand.

Accreditation

Royal Society of Chemistry Accreditation

The University of Leeds launched the first taught MSc degree in Chemical Biology in the UK. The course was one of the first two MSc courses in the UK to receive accreditation from the Royal Society of Chemistry; graduates from the programme with an appropriate first degree in chemistry satisfy the academic requirements for the award of Chartered Chemist (CChem) status.



Read less

  • 1
Show 10 15 30 per page



Cookie Policy    X