• University of Derby Online Learning Featured Masters Courses
  • Jacobs University Bremen gGmbH Featured Masters Courses
  • University of Bristol Featured Masters Courses
  • Aberystwyth University Featured Masters Courses
  • Northumbria University Featured Masters Courses
  • University of Edinburgh Featured Masters Courses
  • University of Leeds Featured Masters Courses

Postgrad LIVE! Study Fair

Birmingham | Bristol | Sheffield | Liverpool | Edinburgh

Cranfield University Featured Masters Courses
Nottingham Trent University Featured Masters Courses
Southampton Solent University Featured Masters Courses
Xi’an Jiaotong-Liverpool University Featured Masters Courses
Swansea University Featured Masters Courses

Masters Discussion Forum

The following thread is brought to you by our sister Web site PostgraduateForum.com. If you wish to reply or post your own thread, you will be redirected to this site.

This Category:   PostgraduateForum.com > Research Methods


IC50 value problem in Graphpad prism

User: SonjaB - 11 September 2017 15:29

Hello guys,

I am struggling with my data analysis in graphpad prism and looking for some help of advanced people.

I did a cell viability assay and plotted my datas in graphpad prism to calculate the IC50 value.

The curves I got look very different from each other and the calculated IC50 value seems not to represent the real values.

I draw some lines in my graph to get an idea of the real IC50 value and compared it to the ones from the table, however for one of my curves it seems much lower as the true value.

When setting the top to the last value of my curve, then it gives me the real value, however when doing so, it also sets this top for the other curve, which gives me then wrong value for this curve.

Is there a way to contraint the top only for one curve?
If you need more information or want to see the curve to get an idea of the problem please text me and I can try to send a image of the graph.

Thank you so much for help

Cookie Policy    X